2026
Marieke A.J. Hof, Jeffrey Sewdihal, Stepan Stepanović, Jos H. Hermans, Péter Horvatovich, Peter Olinga, Frank Klont. Inter-species variability in 4,4′-methylenedianiline metabolism: insights from human and rat precision-cut liver slices. Regulatory Toxicology and Pharmacology 2026; 166: 106034.
2025
Mugdha M. Joglekar, Jannie M.B. Sand, Theo Borghuis, Diana Julie Leeming, Morten Karsdal, Frank Klont, Russell P. Bowler, Barbro N. Melgert, Janette K. Burgess, Simon D. Pouwels. The calprotectin fragment, CPa9-HNE, is a plasma biomarker of mild chronic obstructive pulmonary disease. Cells 2025; 14: 1155.
Marieke A.J. Hof & Hessel de Haan & Stepan Stepanović, Stephan J.L. Bakker, Eelko Hak, Gérard Hopfgartner, Frank Klont, TransplantLines Investigators. Pharmacometabolomics study of sulfamethoxazole and trimethoprim in kidney transplant recipients: real-world metabolism and urinary excretion. Metabolites 2025; 15: 473.
Fleur B. Nijdam, Marieke A.J. Hof, Daan Kremer, Tim J. Knobbe, Gérard Hopfgartner, Stephan J.L. Bakker, Eelko Hak, Frank Klont, TransplantLines Investigators. Dose, kidney function, and a drug-excipient interaction impair mycophenolate mofetil prodrug activation in kidney transplant recipients. European Journal of Drug Metabolism and Pharmacokinetics 2025; 50: 341-352.
Wietske A. Heddema, Marieke A.J. Hof, Piotr Sosnowski, Stephan J.L. Bakker, Gérard Hopfgartner, Frank Klont, TransplantLines Investigators. Pharmacometabolomics detects various unreported metoprolol metabolites in urine of (potential) living kidney donors and kidney transplant recipients. Clinical Pharmacokinetics 2025; 64: 779-789.
Frank Klont, J. Stein Bellinga, Valentina Giunchi, Spyros Balafas, Eelko Hak. A pharmacological view on accounting for pharmacokinetic factors in the estimation of pharmaceutical emissions based on drug usage data. Environment International 2025; 196: 109312.
Fleur B. Nijdam, Marieke A.J. Hof, Hans Blokzijl, Stephan J.L. Bakker, Eelko Hak, Gérard Hopfgartner, Frank Klont, TransplantLines Investigators. Pharmacometabolomics enables real-world drug metabolism sciences. Metabolites 2025; 15: 39.
2024
Frank Klont, Marieke A.J. Hof, Fleur B. Nijdam, Daan J. Touw, Stephan J.L. Bakker, Gérard Hopfgartner, Jos G.W. Kosterink, Eelko Hak. Pharmacometabolomics may be the next stamp in the pharmacogenetic passport. Pharmacological Research 2024; 204: 107191.
Frank Klont, Fleur B. Nijdam, Stephan J.L. Bakker, Pekka Keski-Rahkonen, Gérard Hopfgartner, TransplantLines Investigators. High-abundance peaks and peak clusters associate with pharmaceutical polymers and excipients in urinary untargeted clinical metabolomics data: exploration of their origin and possible impact on label-free quantification. Analyst 2024; 149: 1061-1067.
2023
Tim J. Knobbe, Daan Kremer, Rianne M. Douwes, Michele F. Eisenga, António W. Gomes-Neto, Coby Annema, J. Casper Swarte, Frank Klont, Gerjan Navis, Stefan P. Berger, Stephan J.L. Bakker, TransplantLines Investigators. Proton pump inhibitor use, fatigue, and health-related quality of life in kidney transplant recipients: results from the TransplantLines Biobank and Cohort Study. American Journal of Kidney Diseases 2023; 82: 189-201.
Frank Klont, Oladapo Olaleye, Rainer Bischoff. Quantification of proteins in blood by absorptive microtiter plate-based affinity purification coupled to liquid chromatography-mass spectrometry. Methods in Molecular Biology 2023; 2628: 221-233.
2022
Frank Klont, Piotr Sosnowski, Daan Kremer, Tim J. Knobbe, Ron Bonner, Hans Blokzijl, Rinse K. Weersma, Stephan J.L. Bakker, TransplantLines Investigators, Eelko Hak, Daan J. Touw, and Gérard Hopfgartner. Assessing the Potential of Untargeted SWATH Mass Spectrometry-Based Metabolomics to Differentiate Closely Related Exposures in Observational Studies. Metabolites 2022; 12: 942.
Frank Klont, Stepan Stepanović, Daan Kremer, Ron Bonner, Daan J. Touw, Eelko Hak, Stephan J.L. Bakker, Gérard Hopfgartner. Untargeted ‘SWATH’ mass spectrometry-based metabolomics for studying chronic and intermittent exposure to xenobiotics in cohort studies. Food and Chemical Toxicology 2022; 165: 113188.
Sara Sokooti, Frank Klont, Sok Cin Tye, Daan Kremer, Rianne M. Douwes, Gérard Hopfgartner, Robin P.F. Dullaart, Hiddo J.L. Heerspink, Stephan J.L. Bakker. Association of diuretic use with increased risk for long-term post-transplantation diabetes mellitus in kidney transplant recipients. Nephrology Dialysis Transplantation 2022; 37: 1375-1383.
Frank Klont, Péter Horvatovich, Russell P. Bowler, Eva van Rikxoort, Jean-Paul Charbonnier, Marcel Kwiatkowski, David A. Lynch, Stephen Humphries, Rainer Bischoff, Nick H.T. ten Hacken, Simon D. Pouwels. Plasma sRAGE levels strongly associate with centrilobular emphysema assessed by HRCT scans. Respiratory Research 2022; 23: 15.
2021
Frank Klont, Gérard Hopfgartner. Mass spectrometry based approaches and strategies in bioanalysis for qualitative and quantitative analysis of pharmaceutically relevant molecules. Drug Discovery Today: Technologies 2021; 40: 64-68.
Frank Klont, Marcel Kwiatkowski, Alen Faiz, Thea van den Bosch, Simon D. Pouwels, Frank J. Dekker, Nick H.T. ten Hacken, Péter Horvatovich, Rainer Bischoff. Adsorptive Microtiter Plates As Solid Supports in Affinity Purification Workflows. Journal of Proteome Research 2021; 20: 5218-5221.
David Ruskic, Frank Klont, Gérard Hopfgartner. Clustering and nonclustering modifier mixtures in differential mobility spectrometry for multidimensional liquid chromatography ion mobility–mass spectrometry analysis. Analytical Chemistry 2021; 93: 6638-6645.
Katherine A. Pratte, Jeffrey L. Curtis, Katerina Kechris, David Couper, Michael H. Cho, Edwin K. Silverman, Dawn L. DeMeo, Frank C. Sciurba, Yingze Zhang, Victor E. Ortega, Wanda K. O’Neal, Lucas A. Gillenwater, David A. Lynch, Eric A. Hoffman, John D. Newell Jr., Alejandro P. Comellas, Peter J. Castaldi, Bruce E. Miller, Simon D. Pouwels, Nick H.T. ten Hacken, Rainer Bischoff, Frank Klont, Prescott G. Woodruff, Robert Paine, R. Graham Barr, John Hoidal, Claire M. Doerschuk, Jean-Paul Charbonnier, Ruby Sung, Nicholas Locantore, John G. Yonchuk, Sean Jacobson, Ruth Tal-Singer, Debbie Merrill, Russell P. Bowler. Soluble receptor for advanced glycation end products (sRAGE) as a biomarker of COPD. Respiratory Research 2021; 22: 127.
Frank Klont, Daan Kremer, Antonio W. Gomes-Neto, Stefan P. Berger, Daan J. Touw, Eelko Hak, Ron Bonner, Stephan J.L. Bakker, Gérard Hopfgartner. Metabolomics data complemented drug use information in epidemiological databases: pilot study of potential kidney donors. Journal of Clinical Epidemiology 2021; 135: 10-16.
2020
Frank Klont, Gérard Hopfgartner. Bioanalytical research and training in academia during the COVID-19 pandemic. Bioanalysis 2020; 12: 1209-1211.
Frank Klont, Péter Horvatovich, Nick H.T. ten Hacken, Rainer Bischoff. Cigarette smoking prior to blood sampling acutely affects serum levels of the chronic obstructive pulmonary disease biomarker surfactant protein D. Clinical Chemistry and Laboratory Medicine 2020; 58: e138-e141.
Frank Klont & Sandra Jahn, Chantal Grivet, Stefan König, Ron Bonner, Gérard Hopfgartner. SWATH data independent acquisition mass spectrometry for screening of xenobiotics in biological fluids: Opportunities and challenges for data processing. Talanta 2020; 211: 120747.
Frank Klont, Lyanne M. Kieneker, Antonio W. Gomes-Neto, Suzanne P. Stam, Nick H.T. ten Hacken, Ido P. Kema, André P. van Beek, Else van den Berg, Péter Horvatovich, Rainer Bischoff, Stephan J.L. Bakker. Female Specific Association of Low Insulin-Like Growth Factor 1 (IGF1) Levels with Increased Risk of Premature Mortality in Renal Transplant Recipients. Journal of Clinical Medicine 2020; 9: 293.
2019
Frank Klont, Simon D. Pouwels, Peter Bults, Nico C. van de Merbel, Nick H.T. ten Hacken, Péter Horvatovich, Rainer Bischoff. Quantification of surfactant protein D (SPD) in human serum by liquid chromatography-mass spectrometry (LC-MS). Talanta 2019; 202: 507-523.
Simon D. Pouwels, Frank Klont, Rainer Bischoff, Nick H.T. ten Hacken. Confounding Factors Affecting sRAGE as Biomarker for COPD. American Journal of Respiratory and Critical Care Medicine 2019; 200: 114.
Simon D. Pouwels & Frank Klont, Marcel D. Kwiatkowski, Valerie R. Wiersma, Alen Faiz, Corneel Vermeulen, Victor Guryev, Maarten van den Berge, Péter Horvatovich, Rainer Bischoff, Nick H.T. ten Hacken. Reply to Biswas: Acute and Chronic Effects of Cigarette Smoking on sRAGE. American Journal of Respiratory and Critical Care Medicine 2019; 199: 806-807.
Frank Klont, Péter Horvatovich, Natalia Govorukhina, Rainer Bischoff. Pre- and Post-analytical Factors in Biomarker Discovery. Methods in Molecular Biology 2019; 1959: 1-22.
2018
Frank Klont, Marc R. Joosten, Péter Horvatovich, Nick H.T. ten Hacken, Rainer Bischoff. Quantification of the Soluble Receptor of Advanced Glycation End-Products (sRAGE) by LC-MS after Enrichment by Strong Cation Exchange (SCX) Solid-Phase Extraction (SPE) at the Protein Level. Analytica Chimica Acta 2018; 1043: 45-52.
Simon D. Pouwels & Frank Klont, Marcel D. Kwiatkowski, Valerie R. Wiersma, Alen Faiz, Corneel Vermeulen, Victor Guryev, Maarten van den Berge, Péter Horvatovich, Rainer Bischoff, Nick H.T. ten Hacken. Cigarette Smoking Acutely Decreases Serum Levels of the COPD Biomarker sRAGE. American Journal of Respiratory and Critical Care Medicine 2018; 198: 1456-1458.
Frank Klont, Marrit Hadderingh, Péter Horvatovich, Nick H.T. ten Hacken, Rainer Bischoff. Affimers as an Alternative to Antibodies in an Affinity LC-MS Assay for Quantification of the Soluble Receptor of Advanced Glycation End-Products (sRAGE) in human serum. Journal of Proteome Research 2018; 17: 2892-2899.
Kees J. Bronsema, Frank Klont, Frank B. Schalk, Rainer Bischoff, Ido P. Kema, Nico C. van de Merbel. A Quantitative LC-MS/MS Method for Insulin-Like Growth Factor 1 in Human Plasma. Clinical Chemistry and Laboratory Medicine 2018; 56: 1905-1912.
Frank Klont, Linda Bras, Justina C. Wolters, Sara Ongay, Rainer Bischoff, Gyorgy B. Halmos, Péter Horvatovich. Assessment of Sample Preparation Bias in Mass Spectrometry-Based Proteomics. Analytical Chemistry 2018; 90: 5405-5413.
Frank Klont & Simon D. Pouwels, Jos Hermans, Nico C. van de Merbel, Péter Horvatovich, Nick H.T. ten Hacken, Rainer Bischoff. A Fully Validated Liquid Chromatography-Mass Spectrometry Method for the Quantification of the Soluble Receptor of Advanced Glycation End-Products (sRAGE) in Serum Using Immunopurification in a 96-Well Plate Format. Talanta 2018; 182: 414-421.
Frank Klont. Demoralization and Stress — We Can All Help (?). Occupational Medicine 2018; 68: 17.
2017
Frank Klont, Nick H.T. ten Hacken, Péter Horvatovich, Stephan J.L. Bakker, Rainer Bischoff. Assuring Consistent Performance of an Insulin-Like Growth Factor 1 MALDImmunoassay by Monitoring Measurement Quality Indicators. Analytical Chemistry 2017; 89: 6188-6195.
2016
Sara Ongay Camacho, Frank Klont, Peter Horvatovich, Rainer Bischoff, Nick H. T. ten Hacken. Prioritization of COPD Protein Biomarkers, Based on a Systematic Study of the Literature. Advances in Precision Medicine 2016; 1: 12-24.
Hink Boer, Nico-Derk L. Westerink, Renske Altena, Janine Nuver, D.A. Janneke Dijck-Brouwer, Martijn van Faassen, Frank Klont, Ido P. Kema, Joop D. Lefrandt, Nynke Zwart, H. Marike Boezen, Andries J. Smit, Coby Meijer, Jourik A. Gietema. Single-Nucleotide Polymorphism in the 5-α-Reductase Gene (SRD5A2) is Associated with Increased Prevalence of Metabolic Syndrome in Chemotherapy-Treated Testicular Cancer Survivors. European Journal of Cancer 2016; 54: 104-111.
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